Wnt/beta-catenin signaling pathway regulates beta1,4-galactosyltransferase l expression in the endometrium to affect the embryo implantation

Successful implantation depends on two aspects: blastocyst invasion ability and endometrial receptivity. β1,4-GalT-I plays an important role in fertilization, cell adhesion, neurite growth and tumor cell migration process. To explore the impact of β1,4-GalT-I in the process of embryo implantation and study on regulation of β1,4-GalT-I expression by Wnt/β-catenin signaling pathway. We established mouse model of early pregnancy, taking D4 pregnant mice uterine horn injected β1,4-GalT-I polyclonal antibody to detect the impact on blastocyst implantation and used reverse immunohistochemistry for detecting β1,4-GalT-I antibody effectiveness. Established in vitro implantation model, using immunofluorescence, Western-blot and cells adhesion experiments to detect whether Wnt/β-catenin signaling pathway regulated β1,4-GalT-I. We found that side of the uterine cavity of pregnant mice injected β1,4GalT-I antibody, the number of embryo implantation (3.3±0.4) compared with the other side injected with saline embryo implantation count (10.1±1.7) was statistically significant (P<0.05). Immunohistochemically staining showed that brown particles precipitate on endometrial cells. Regulation of Wnt/β-catenin signaling pathway may regulate the expression of β1,4-GalT-I. Raised β-catenin expression in RL95-2 cells, the adhesion rate of JAR to RL95-2 cells (85.7±3.3%) was significantly higher compared with non-transfected group (53.6±2.1%). However, reduced β-catenin expression in RL95-2 cells, the adhesion rate of JAR to RL95-2 cells (35.3±1.6%) declined. β1,4-GalT-I participate in the process of embryo implantation. β1,4-GalT-I expression in endometrium during implantation and Wnt/β-catenin signaling pathway molecule expression has correlation and it may be located in Wnt/β-catenin signaling pathway downstream. In short, Wnt/β-catenin signaling pathway may regulate β1,4-GalT-I expression in the endometrium to involve the adhesion of JAR to RL95-2 cells.